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Phoenix Pharmaceuticals globular adiponectin (adn)
( A ) Representative normalized Bmal1-luc luminescence rhythms of <t>dexamethasone</t> (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .
Globular Adiponectin (Adn), supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/globular+adiponectin+(adn)/recombinant+globular+adiponectin/pmc07375813-256-2-12
Average 90 stars, based on 1 article reviews
globular adiponectin (adn) - by Bioz Stars, 2026-09
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1) Product Images from "An adipokine feedback regulating diurnal food intake rhythms in mice"

Article Title: An adipokine feedback regulating diurnal food intake rhythms in mice

Journal: eLife

doi: 10.7554/eLife.55388

( A ) Representative normalized Bmal1-luc luminescence rhythms of dexamethasone (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .
Figure Legend Snippet: ( A ) Representative normalized Bmal1-luc luminescence rhythms of dexamethasone (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .

Techniques Used: Derivative Assay

( A, B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with dexamethasone (Dex; A ) or forskolin (Fors; B ) or PBS at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Bottom panels: phase response curves for dexamethasone (left) and forskolin (right). Shown are averages of three experiments per time point each with sine wave regressions (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see for reference.
Figure Legend Snippet: ( A, B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with dexamethasone (Dex; A ) or forskolin (Fors; B ) or PBS at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Bottom panels: phase response curves for dexamethasone (left) and forskolin (right). Shown are averages of three experiments per time point each with sine wave regressions (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see for reference.

Techniques Used:



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Phoenix Pharmaceuticals globular adiponectin (adn)
( A ) Representative normalized Bmal1-luc luminescence rhythms of <t>dexamethasone</t> (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .
Globular Adiponectin (Adn), supplied by Phoenix Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/globular+adiponectin+(adn)/recombinant+globular+adiponectin/pmc07375813-256-2-12
Average 90 stars, based on 1 article reviews
globular adiponectin (adn) - by Bioz Stars, 2026-09
90/100 stars
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( A ) Representative normalized Bmal1-luc luminescence rhythms of dexamethasone (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .

Journal: eLife

Article Title: An adipokine feedback regulating diurnal food intake rhythms in mice

doi: 10.7554/eLife.55388

Figure Lengend Snippet: ( A ) Representative normalized Bmal1-luc luminescence rhythms of dexamethasone (DEX) synchronized hypothalamus-derived mHypo-N44 ( N44 ) cells. Inset depicts raw luminescence data of the same set. ( B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with ADIPOQ (Adn; red and blue) peptide or PBS (black) at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. ( C ) Phase response curve for ADIPOQ-mediated resetting of N44/ Bmal1-luc cells (n = 3 per time point; treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( A ) for reference). ( D ) Normalized PER2::LUC luminescence rhythms of primary hypothalamic neurons after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Inset shows quantification of phase shifts (p<0.0001; unpaired Student’s t-test). ( E ) Normalized PER2::LUC luminescence rhythms of organotypic MBH slices after treatment with ADIPOQ (Adn; red and blue) peptide at the depicted time points. Shown are averages ± SEM of 3 traces each. ( F ) Phase response curve for ADIPOQ-mediated resetting of PER2::LUC MBH slices (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see ( E ) for reference. Figure 5—source data 1. Raw data of experiments shown in .

Article Snippet: Forskolin (Fors), dexamethasone (Dex), globular adiponectin (Adn), and AdipoRon were purchased from Phoenix Pharmaceuticals (Karlsruhe, DE); leptin and full-length adiponectin were purchased from Enzo Life Science (Loerrach, DE); ghrelin, glucagon, and nesfatin were purchased from Bachem (Bubendorf, CH); resistin was purchased from Prospec (NJ, USA) and visfatin from Aviscera Bioscience (CA, USA); VPR66 was purchased from BioMol (Hamburg, DE).

Techniques: Derivative Assay

( A, B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with dexamethasone (Dex; A ) or forskolin (Fors; B ) or PBS at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Bottom panels: phase response curves for dexamethasone (left) and forskolin (right). Shown are averages of three experiments per time point each with sine wave regressions (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see for reference.

Journal: eLife

Article Title: An adipokine feedback regulating diurnal food intake rhythms in mice

doi: 10.7554/eLife.55388

Figure Lengend Snippet: ( A, B ) Normalized Bmal1-luc luminescence rhythms of N44 cells after treatment with dexamethasone (Dex; A ) or forskolin (Fors; B ) or PBS at the depicted time points after synchronization. Shown are averages ± SEM of 3 traces each. Bottom panels: phase response curves for dexamethasone (left) and forskolin (right). Shown are averages of three experiments per time point each with sine wave regressions (treatment time given in degrees with 90 °=maximal luminescence and 270 °=minimal luminescence – see for reference.

Article Snippet: Forskolin (Fors), dexamethasone (Dex), globular adiponectin (Adn), and AdipoRon were purchased from Phoenix Pharmaceuticals (Karlsruhe, DE); leptin and full-length adiponectin were purchased from Enzo Life Science (Loerrach, DE); ghrelin, glucagon, and nesfatin were purchased from Bachem (Bubendorf, CH); resistin was purchased from Prospec (NJ, USA) and visfatin from Aviscera Bioscience (CA, USA); VPR66 was purchased from BioMol (Hamburg, DE).

Techniques: